Mouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability
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mouse oocytes and embryos cryotop-vitrification using low concentrated solutions: effects on meiotic spindle, genetic material array and developmental ability
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full textMouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability
OBJECTIVE(S) The examination of the possibility of applying lower CPA- concentrations and obtaining the similar results to those using higher concentrations; as it is shown, the toxicity of the CPAs used in vitrification approach will diminish. MATERIALS AND METHODS Following vitrification/warming, oocytes were subjected to PZD/ICSI. SRs, FRs, and DRs were recorded. SRs and DRs of the embryos...
full textP-132: The Effect of Vitrification on Mouse Oocytes Survivale Rate and Apoptosis Using Cryotop
Background: Vitrification of oocyte is one of the most important topics in the field of assisted reproductive techniques (ART). Considering the importance of oocyte vitrification in clinics, in the present study the effect of vitrification on mouse oocytes survivale rate and apoptosis by cryotop were investigated. Materials and Methods: 200 GV oocytes and 200 MII oocytes were obtained respectiv...
full textComparison of The Effects of Vitrification on Gene Expression of Mature Mouse Oocytes Using Cryotop and Open Pulled Straw
Objective Oocyte cryopreservation is an essential part of assisted reproductive technology (ART) which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems (Cryotop and Open Pulled Straw, OPS) on mature oocytes gene expressions. MaterialsAndMethods The survival rate of metaphase II (MII) mouse oocytes were assessed after cryoprese...
full textVitrification by Cryotop and the Maturation, Fertilization, and Developmental Rates of Mouse Oocytes
BACKGROUND Oocyte cryopreservation is an important part of modern fertility treatment. The effect of vitrification on the fertilization and developmental rates of embryo is still a matter of debate. OBJECTIVES This study aimed to investigate the effect of vitrification on the success of mouse oocyte maturation, fertilization, and preimplantation development in vitro. MATERIALS AND METHODS I...
full textdevelopmental consequences of mouse cryotop-vitrified oocyte and embryo using low concentrated cryoprotectants
background: the risk of multiple pregnancies often present in programs of in vitro fertilization (ivf) is an important force for embryo cryopreservation. on the other hand ethical restriction and assurance of potential fertility following chemo/radio therapy has led scientists to focus on female gamete preservation. objective: optimizing vitrification protocol by using less concentrated cryopro...
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Journal title
volume 16 issue 4
pages 599- 609
publication date 2013-04-01
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